Anti-BRTC1/2 (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q9Y297
Gene Id	8945
Gene Symbols	BTRC
Protein Name / Synonyms	BRTC1/2 (N-term)
Clonality	Polyclonal
Preparation	This BRTC1/2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 127-156 amino acids from the N-terminal region of human BRTC1/2.
Concentration (lot specific)	0.340 mg/ml
Recommended Applications	Western Blotting
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds to phosphorylated target proteins. SCF(BTRC) mediates the ubiquitination of CTNNB1 and participates in Wnt signaling. SCF(BTRC) mediates the ubiquitination of NFKBIA, NFKBIB and NFKBIE; the degradation frees the associated NFKB1 to translocate into the nucleus and to activate transcription. Ubiquitination of NFKBIA occurs at 'Lys-21' and 'Lys-22'. SCF(BTRC) mediates the ubiquitination of phosphorylated NFKB1/nuclear factor NF-kappa-B p105 subunit, ATF4, SMAD3, SMAD4, CDC25A, DLG1, FBXO5 and probably NFKB2. SCF(BTRC) mediates the ubiquitination of phosphorylated SNAI1. May be involved in ubiquitination and subsequent proteasomal degradation through a DBB1-CUL4 E3 ubiquitin-protein ligase. Required for activation of NFKB-mediated transcription by IL1B, MAP3K14, MAP3K1, IKBKB and TNF. Required for proteolytic processing of GLI3.

Specificity

Predicted Reactivity

Mouse

Other Names

BTRC;BTRCP; FBW1A; FBXW1A; F-box/WD repeat-containing protein 1A; F-box/WD repeat-containing protein 1A; E3RSIkappaB; F-box/WD repeat-containing protein 1A; Epididymis tissue protein Li 2a; F-box/WD repeat-containing protein 1A; F-box and WD repeats protein beta-TrCP; F-box/WD repeat-containing protein 1A; pIkappaBalpha-E3 receptor subunit

NCBI Accession #

NP_003930.1;NP_378663.1

Other Accession #

Q3ULA2

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

68867

Recommended Dilutions

WB: 1:1000

BRTC1/2 Antibody (N-term) (Cat# 102-14823) western blot analysis in MDA-MB453,HepG2 cell line lysates (35ug/lane).This demonstrates the BRTC1/2 antibody detected the BRTC1/2 protein (arrow).

Antigen Source

Human

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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