Anti-BUBR1 (BUB1B) (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	O60566
Gene Id	701
Gene Symbols	BUB1B
Protein Name / Synonyms	Mitotic checkpoint serine/threonine-protein kinase BUB1 beta, MAD3/BUB1-related protein kinase, hBUBR1, Mitotic checkpoint kinase MAD3L, Protein SSK1, BUB1B, BUBR1, MAD3L, SSK1
Clonality	Polyclonal
Clone	RB03121-03122
Isotype	Kappa, Rabbit
Recommended Applications	Western Blotting, IHC-P
Research Area	Cancer
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

BUB1B, a member of the Ser/Thr protein kinase family, is a probable component of the mitotic checkpoint that delays anaphase until all chromosomes are properly attached to the mitotic spindle. It can interact with BUB3, CENP-F, CENP-E and mitosin, and can be localized to nuclear kinetochores. This protein is highly expressed in thymus and spleen. The CD1 domain directs kinetochore localization and binding to BUB3. The protein possesses a cyclin destruction box sequence, which targets protein for rapid degradation by ubiquitin-dependent proteolysis during the transition from mitosis to interphase. Defects in BUB1B are associated with tumor formation.

Other Information

NCBI Accession #

NP_001202.4

Antigen Type

Synthetic Peptide

Format

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Calculated Molecular Weight (Da)

119545

Antigen Source

Human

Images

WB (1:1000)

Western blot analysis of BUB1B (arrow) using rabbit polyclonal BUB1B Antibody (N-term) (Cat.#102-27483). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BUB1B gene (Lane 2) (Origene Technologies).

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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