Synonyms
AHUS5 antibody, ARMD9 antibody, ASP antibody, CPAMD1 antibody, C3 antibody, complement C3 antibody, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1 antibody, complement component C3 antibody, acylation-stimulating protein cleavage product antibody, complement component 3 antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
187 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1448 and 1655 of (Uniprot ID# P01024)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:5000-1:20000, IHC-P: 1:100-1:1000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using C3(107-10254) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded mouse brain, using C3(107-10254) antibody at 1:500 dilution.

C3 antibody [C3], C-term detects C3 protein by western blot analysis.
A. 30 µg 293T whole cell extract
B. 30 µg whole cell extract of human C3-transfected 293T cells
5 % SDS-PAGE
C3 antibody [C3], C-term (107-10254) dilution: 1:20000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.