Anti-C5 (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P01031
Gene Id	727
Gene Symbols	C5
Protein Name / Synonyms	C5 (N-term)
Clonality	Polyclonal
Preparation	This C5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 253-281 amino acids from the N-terminal region of human C5.
Recommended Applications	Western Blotting, IHC-P, FC
Research Area	Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

C5 is the fifth component of complement, which plays an important role in inflammatory and cell killing processes. This protein is comprised of alpha and beta polypeptide chains that are linked by a disulfide bridge. An activation peptide, C5a, which is an anaphylatoxin that possesses potent spasmogenic and chemotactic activity, is derived from the alpha polypeptide via cleavage with a convertase. The C5b macromolecular cleavage product can form a complex with the C6 complement component, and this complex is the basis for formation of the membrane attack complex, which includes additional complement components.

Specificity

Other Names

C5; CPAMD4; Complement C5; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4; Complement C5 beta chain; Complement C5 alpha chain; C5a anaphylatoxin; Complement C5 alpha' chain

NCBI Accession #

NP_001726.2

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

188305

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; FC: 1:10-50

	Western blot analysis of C5 Antibody (N-term) (Cat# 102-17926) in HL-60 cell line lysates (35ug/lane). C5 (arrow) was detected using the purified Pab.(2ug/ml)
	Formalin-fixed and paraffin-embedded human breast carcinoma with C5 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Flow cytometric analysis of MDA-231 cells using C5 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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