Synonyms
FLJ23305 antibody, bA282P11.1 antibody, C6orf97 antibody, coiled-coil domain-containing protein C6orf97 antibody, chromosome 6 open reading frame 97 antibody
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
82 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 442 and 715 of LOC80129 (Uniprot ID# Q8IYT3)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of methanol-fixed HeLa, using LOC80129(107-10670) antibody at 1:200 dilution.

C6orf97 antibody detects C6orf97 protein at cytosol on BT483 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded BT483 xenograft.
C6orf97 antibody (107-10670) dilution: 1:500.

C6orf97 antibody detects C6orf97 protein at cytosol on gastric carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded gastric carcinoma.
C6orf97 antibody (107-10670) dilution: 1:500.

Sample (30 ug of whole cell lysate)
A: A549
7.5% SDS PAGE
107-10670 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.