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Anti-CA2 (N-term) Antibody
Catalog #: 102-17261
Rabbit Anti-CA2 (N-term) Antibody, 400 ul
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P00918 |
| Gene Id | 760 |
| Gene Symbols | CA2 |
| Protein Name / Synonyms | CA2 (N-term) |
| Clonality | Polyclonal |
| Preparation | This CA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 54-83 amino acids from the N-terminal region of human CA2. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
CA2 is one of several (at least 7) isozymes of carbonic anhydrase. Carbonic anhydrase catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis.
Specificity
Other Names
CA2; Carbonic anhydrase 2; Carbonate dehydratase II; Carbonic anhydrase C; Carbonic anhydrase II
NCBI Accession #
NP_000058.1
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
29246
Recommended Dilutions
WB: 1:1000; IF: 1:10-50
 | Western blot analysis of lysates from MCF-7 cell line and mouse kidney tissue lysate (from left to right), using CA2 Antibody (N-term) (Cat# 102-17261). 102-17261 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane. |
 | CA2 Antibody (N-term) (Cat# 102-17261) western blot analysis in MCF-7 cell line and mouse kidney tissue lysates (35ug/lane).This demonstrates the CA2 antibody detected the CA2 protein (arrow). |
 | Fluorescent confocal image of Hela cell stained with CA2 Antibody (N-term)(Cat# 102-17261).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CA2 primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). CA2 immunoreactivity is localized to Cytoplasm significantly. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.