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Anti-CA9 (Center) Antibody
Catalog #: 102-15840
Rabbit Anti-Human CA9 (Center) Antibody, 400 ul
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q16790 |
| Gene Id | 768 |
| Gene Symbols | CA9 |
| Protein Name / Synonyms | CA9 (Center) |
| Clonality | Polyclonal |
| Preparation | This CA9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 113-143 amino acids from the Central region of human CA9. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, FC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation.
Specificity
Other Names
CA9; G250; MN; Carbonic anhydrase 9; Carbonate dehydratase IX; Carbonic anhydrase IX; Membrane antigen MN; P54/58N; Renal cell carcinoma-associated antigen G250; pMW1
NCBI Accession #
NP_001207.2
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
49698
Recommended Dilutions
WB: 1:1000; FC: 1:10-50; IF: 1:10-50
 | Western blot analysis of CA9 antibody (Center) (Cat# 102-15840) in Hela cell line lysates (35ug/lane). CA9 (arrow) was detected using the purified Pab. |
 | Flow cytometric analysis of hela cells using CA9 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
 | Immunofluorescence analysis of CA9 Antibody (Center) with hela cells . 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.