Anti-CAF-1 (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q9UIV1
Gene Id	29883
Gene Symbols	CNOT7
Protein Name / Synonyms	CAF-1 (N-term)
Clonality	Polyclonal
Preparation	This CAF-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 34-61 amino acids from the N-terminal region of human CAF-1.
Concentration (lot specific)	0.310 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

The protein encoded by this gene binds to an anti-proliferative protein, B-cell translocation protein 1, which negatively regulates cell proliferation. Binding of the two proteins, which is driven by phosphorylation of the anti-proliferative protein, causes signaling events in cell division that lead to changes in cell proliferation associated with cell-cell contact. The protein has both mouse and yeast orthologs. Alternate splicing of this gene results in two transcript variants encoding different isoforms.

Specificity

Predicted Reactivity

Mouse, Zebrafish, Bovine, Chicken, Xenopus

Other Names

CNOT7; CAF1; CCR4-NOT transcription complex subunit 7; BTG1-binding factor 1; CCR4-associated factor 1

NCBI Accession #

NP_037486.2;NP_473367.2

Other Accession #

Q3KQ85; Q60809; Q08BM8; Q5ZJV9; Q3ZC01; NP_473367.2

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

32745

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; IF: 1:10-50

	CAF-1 Antibody (N-term) (Cat# 102-10944) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the CAF-1 antibody detected the CAF-1 protein (arrow).
	CAF-1 Antibody (N-term) (Cat# 102-10944)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CAF-1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
	Fluorescent confocal image of Hela cell stained with CAF-1 Antibody (N-term)(Cat# 102-10944).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CAF-1 primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). CAF-1 immunoreactivity is localized to Cytoplasm and Nucleus significantly.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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