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Anti-Calpain-5 Antibody
Catalog #: 107-10377
Calpain-5 antibody [N1C1], 0.47 mg/ml (100 ul)
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 726 |
| Protein Name / Synonyms | Calpain-5 |
| Concentration (lot specific) | 0.47 mg/ml |
| Recommended Applications | ICC/IF, Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Synonyms
FLJ46245 antibody, HTRA3 antibody, nCL-3 antibody, CAPN5 antibody, calpain htra-3 antibody, calpain-5 antibody, new calpain 3 antibody, calpain 5 antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
73 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 381 of Calpain-5 (Uniprot ID# O15484)
Applications
Applications Tested
ICC/IF,Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Calpain-5(107-10377) antibody at 1:100 dilution.
Sample (30 ug of whole cell lysate)
A: A431
B: H1299
7.5% SDS PAGE
Calpain-5 antibody
107-10377 diluted at 1:1000
A: A431
B: H1299
7.5% SDS PAGE
Calpain-5 antibody
107-10377 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.