Anti-Calsequestrin-2 Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Gene Id	845
Protein Name / Synonyms	Calsequestrin-2
Concentration (lot specific)	0.68 mg/ml
Recommended Applications	IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

FLJ26321 antibody, FLJ93514 antibody, PDIB2 antibody, CASQ2 antibody, calsequestrin-2 antibody, "calsequestrin, cardiac muscle isoform antibody", "calsequestrin 2, fast-twitch, cardiac muscle antibody", calsequestrin 2 (cardiac muscle) antibody

Specificity

Species Reactivity

Human

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

46 kDa

Immunogen

Recombinant protein fragment contain a sequence corresponding to a region within amino acids 124 and 388 (O14958) of Calsequestrin 2

Applications

Applications Tested

IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Calsequestrin-2 antibody detects Calsequestrin-2 protein at cytosol on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
Calsequestrin-2 antibody (107-10570) dilution: 1:500.

Calsequestrin 2 antibody detects Calsequestrin 2 protein by western blot analysis.
A. 30 µg HCT116 whole cell lysate/extract
10 % SDS-PAGE
Calsequestrin 2 antibody (107-10570) dilution: 1:1000

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft , using Calsequestrin-2(107-10570) antibody at 1:500 dilution.

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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