Anti-CAMKK2 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q96RR4
Gene Id	10645
Gene Symbols	CAMKK2
Protein Name / Synonyms	CAMKK2 (C-term)
Clonality	Polyclonal
Preparation	This CAMKK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 483-512 amino acids from the C-terminal region of human CAMKK2.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P
Research Area	Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

CAMKK2 belongs to the Serine/Threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This protein plays a role in the calcium/calmodulin-dependent (CaM) kinase cascade by phosphorylating the downstream kinases CaMK1 and CaMK4. Isoform 1, isoform 2 and isoform 3 phosphorylate CAMK1 and CAMK4. Isoform 3 phosphorylates CAMK1D. Isoform 4, isoform 5 and isoform 6 lacking part of the calmodulin-binding domain are inactive. CAMKK2 appears to be involved in hippocampal activation of CREB1.

Specificity

Predicted Reactivity

Mouse, Rat

Other Names

CAMKK2; CAMKKB; KIAA0787; Calcium/calmodulin-dependent protein kinase kinase 2; Calcium/calmodulin-dependent protein kinase kinase beta

NCBI Accession #

NP_001257414.1;NP_001257415.1;NP_006540.3;NP_705719.2;NP_705720.1;NP_757363.1;NP_757364.1;NP_757365.1;NP_757380.1

Other Accession #

O88831; Q8C078

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

64746

Recommended Dilutions

WB: 1:1000; IHC: 1:50

	Western blot analysis of CAMKK2 (arrow) using rabbit polyclonal CAMKK2 Antibody (C-term)(Cat# 102-17138). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CAMKK2 gene (Lane 2) (Origene Technologies).
	Formalin-fixed and paraffin-embedded human brain tissue reacted with CAMKK2 antibody (C-term)(Cat#AP711b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.