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Anti-CAPZA2 Antibody

Catalog #: 144-02054

CAPZA2 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse
Host SpeciesRabbit
Accession Number
P47755
Gene Id
830
Gene Symbols
CAPZA2
Protein Name / Synonyms
CAPZA2, CAPPA2, CAPZ, F-actin-capping protein subunit alpha-2
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IF
Shipping TypeBlue ice
Storage-20°C

Description

Background

The protein encoded by this gene is a member of the F-actin capping protein alpha subunit family. It is the alpha subunit of the barbed-end actin binding protein Cap Z. By capping the barbed end of actin filaments, Cap Z regulates the growth of the actin filaments at the barbed end.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW
19kDa/32kDa
Observed MW
38kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-286 of human CAPZA2 (NP_006127.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using CAPZA2 antibody (144-02054) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - CAPZA2 antibody (144-02054)

Immunofluorescence analysis of HepG2 cells using CAPZA2 antibody (144-02054). Blue: DAPI for nuclear staining.
Immunofluorescence - CAPZA2 antibody (144-02054)

Expiration:
12 months from the date of shipment when stored properly.