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Anti-CART Antibody, Biotinylated
Catalog #: RB-08-0012B-50
Rabbit anti-CART Biotinylated Antibody, 50 ug
Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | CAG47012 |
| Clonality | Polyclonal |
| Immunogen | Immunogen was synthetic peptide derived from Human Cart. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbitt serum by ammonium sulphate precipitation followed by Protein A/G affinity chromatography. |
| Immunogen Sequence | CAVRKGARIGKL |
| Isotype | IgG |
| Recommended Applications | ELISA (recommended work dilution= 1:10e6(at least detecting 2-5 µg/ml)), Western Blotting (recommended work dilution= 1:1000-5000(at least)) |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
The neuropeptide, Cocaine and Amphetamine Regulated Transcript (CART), was identified as an mRNA that changed in response to psychostimulant drug administration and injection of CART peptides into the ventral tegmental area produces psychostimulant-like effects. CART encodes for a protein which has an important role in the regulation of appetite and body weight. CART localizes to areas of the central and peripheral nervous systems and is involved in feeding behavior when injected centrally. Expression of CART in the rat hypothalamus is modulated by nutritional status, and injection of synthetic CART peptide into the forebrain ventricular system suppresses food intake, indicating a possible role in hypothalamic control of energy homeostasis. Its identification in cell bodies and central terminals of vagal afferent neurons additionally suggests a role in brainstem mechanisms of meal termination and satiety.
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
The rabbit anti-human CART antibody detects specifically target derived from human recombinant protein at various concentrations. Cross reactivity with mouse and rat are expected from sequence similarity.
References
- Press DA, Wall MJ. Expression of cocaine- and amphetamine-regulated transcript (CART) peptides at climbing fibre-Purkinje cell synapses in the rat vestibular cerebellum. Neuropeptides. 2008 Feb;42(1):39-46.
- Kozsurek M, et.al.,Cocaine- and amphetamine-regulated transcript peptide (CART) is present in peptidergic C primary afferents and axons of excitatory interneurons with a possible role in nociception in the superficial laminae of the rat spinal cord. Eur J Neurosci. 2007 Sep;26(6):1624-31.
Expiration:
12 months from the date of shipment when stored properly.