Anti-CASK (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	O14936
Gene Id	8573
Gene Symbols	CASK
Protein Name / Synonyms	CASK (C-term)
Clonality	Polyclonal
Preparation	This CASK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 564-596 amino acids from the C-terminal region of human CASK.
Concentration (lot specific)	1.570 mg/ml
Recommended Applications	Western Blotting, IHC-P
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The calcium/calmodulin-dependent kinase (CAMK) group consists of 75 kinases regulated by Ca2+/CaM and close relative family (CAMK, CAMKL, DAPK, MAPKAPK).

Specificity

Predicted Reactivity

Rat

Other Names

CASK; LIN2; Peripheral plasma membrane protein CASK; Calcium/calmodulin-dependent serine protein kinase; Protein lin-2 homolog

NCBI Accession #

NP_001119526.1;NP_001119527.1;NP_003679.2

Other Accession #

Q62915; O70589

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

105123

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100

	Western blot analysis of CASK (arrow) using Cask Antibody (C-term) (Cat# 102-17185). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CASK gene (Lane 2) (Origene Technologies).
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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