Anti-CASP3 (Asp175) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P42574
Gene Id	836
Gene Symbols	CASP3
Protein Name / Synonyms	CASP3(Asp175)
Clonality	Polyclonal
Preparation	This CASP3(Asp175) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 149-179 amino acids from human CASP3(Asp175).
Concentration (lot specific)	0.330 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC
Research Area	Apoptosis, Cancer, Inflammation, Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.

Specificity

Other Names

CASP3; CPP32; Caspase-3; Apopain; Cysteine protease CPP32; Protein Yama; SREBP cleavage activity 1; Caspase-3 subunit p17; Caspase-3 subunit p12

NCBI Accession #

NP_004337.2;NP_116786.1

Other Accession #

NP_004337

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

31608

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; FC: 1:10-50

	Western blot analysis of CASP3 (Asp175) Antibody (Cat# 102-15820) in CEM cell line lysates (35ug/lane). CASP3 (arrow) was detected using the purified Pab.
	CASP3(Asp175) Antibody (Cat# 102-15820) IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CASP3(Asp175) Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
	Western blot analysis of CASP3 (arrow) using rabbit polyclonal CASP3 Antibody (Cat# 102-15820). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CASP3 gene.
	CASP3(Asp175) Antibody (Cat# 102-15820) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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