Anti-CASP8 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q14790
Gene Id	841
Gene Symbols	CASP8
Protein Name / Synonyms	CASP8 (C-term)
Clonality	Polyclonal
Preparation	This CASP8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 432-461 amino acids from the C-terminal region of human CASP8.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, FC
Research Area	Angiogenesis, Apoptosis, Cancer, Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

CASP8 is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases.

Specificity

Other Names

CASP8; MCH5; Caspase-8; Apoptotic cysteine protease; Apoptotic protease Mch-5; CAP4; FADD-homologous ICE/ced-3-like protease; FADD-like ICE; ICE-like apoptotic protease 5; MORT1-associated ced-3 homolog; Caspase-8 subunit p18; Caspase-8 subunit p10

NCBI Accession #

NP_001073593.1;NP_001073594.1;NP_001219.2;NP_203519.1;NP_203520.1;NP_203522.1

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

55391

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50; FC: 1:10-50; IF: 1:10-50

	Western blot analysis of CASP8 antibody (C-term) (Cat# 102-16823) in Hela cell line lysates (35ug/lane). CASP8 (arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with CASP8 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Flow cytometric analysis of hela cells using CASP8 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	Immunofluorescence analysis of CASP8 Antibody (C-term) with hela cells . 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.
	Confocal immunofluorescent analysis of CASP8 Antibody (C-term) (Cat# 102-16823) with Hela cell followed by Alexa Fluor^® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).