Anti-CASP9 (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P55211
Gene Id	842
Gene Symbols	CASP9
Protein Name / Synonyms	CASP9 (Center)
Clonality	Polyclonal
Preparation	This CASP9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 183-211 amino acids from the Central region of human CASP9.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF
Research Area	Apoptosis
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Caspase 9 is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade.

Specificity

Other Names

CASP9; MCH6; Caspase-9; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; ICE-like apoptotic protease 6; Caspase-9 subunit p35; Caspase-9 subunit p10

NCBI Accession #

NP_001220.2;NP_001264983.1;NP_127463.2

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

46281

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50; IF: 1:10-50

	CASP9 Antibody (Center) (Cat# 102-17737) western blot analysis in Hela cell line and mouse L929 tissue lysates (35ug/lane).This demonstrates the CASP9 antibody detected the CASP9 protein (arrow).
	Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CASP9 antibody (Center) (Cat# 102-17737), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Confocal immunofluorescent analysis of CASP9 Antibody (Center)(Cat# 102-17737) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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