Anti-Caspase-3 (CASP3) (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P42574
Gene Id	836
Gene Symbols	CASP3
Protein Name / Synonyms	Caspase-3 (CASP3) (Center)
Clonality	Polyclonal
Preparation	This Caspase-3 (CASP3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 60-90 amino acids from the Central region of human Caspase-3 (CASP3).
Concentration (lot specific)	2.000 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC
Research Area	Apoptosis, Cancer, Inflammation, Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

CASP3 is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease.

Specificity

Other Names

CASP3; CPP32; Caspase-3; Apopain; Cysteine protease CPP32; Protein Yama; SREBP cleavage activity 1; Caspase-3 subunit p17; Caspase-3 subunit p12

NCBI Accession #

NP_004337.2;NP_116786.1

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

31608

Recommended Dilutions

WB: 1:1000; FC: 1:10-50; IHC: 1:10-50

	Western blot analysis of anti-CASP3 Pab (Cat# 102-17420) in NCI-H460 cell line lysates (35ug/lane).CASP3 (arrow) was detected using the purified Pab.
	Western blot analysis of CASP3 (arrow) using rabbit polyclonal CASP3 Antibody (Center) (Cat# 102-17420). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CASP3 gene.
	Flow cytometric analysis of NCI-H460 cells using Caspase-3 (CASP3) Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CASP3 antibody (Center) (Cat# 102-17420), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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