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Anti-Caspase-8 Antibody, Biotinylated
Catalog #: RB-02-0027B-50
Rabbit anti-Caspase-8 Biotinylated Antibody, 50 ug
Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Host Species | Rabbit |
| Accession Number | Q14790 |
| Gene Id | 841 |
| Gene Symbols | CASP8 MCH5 |
| Clonality | Polyclonal |
| Immunogen | The imunogen was synthetic peptide. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum followed by Protein A/G affinity chromatography. |
| Immunogen Sequence | CILSHGDKGIIYGTDGQEAPIYE |
| Isotype | IgG |
| Recommended Applications | ELISA (recommended work dilution= 1: 2, 000) |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Research Area | Angiogenesis, Apoptosis, Cancer, Inflammation |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Apoptosis occurs during normal cellular development and involves dramatic changes in cellular structure. Disruption of apoptosis may contribute to cancer as well as other autoimmune diseases. Caspase family of cysteine proteases has been shown to play a key role in apoptosis. Caspase-8 is a 28 kDa member of the peptidase C14A family of enzymes. It is widely expressed and is considered an initiating caspase for the apoptotic cascade. It serves as a functional subunit, interacting with TRAF2 and activating NF?B. This may account for many of the non-apoptotic activities associated with Caspase-8.
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
This antibody binds to its immunogen, and did not show any cross reactivity with unrelated antigens in ELISA. Cross reactivity with mouse and rat Caspase8 has not yet been tested.
Related Products
- Rabbit Anti-Caspase 8 (CASP8), C-terminus (catalog #: MD-14-1071)
- 2. RayBio® Human Apoptosis Antibody Array G1 (1 slide per kit, 8 sub-arrays per slide), with accessories, for simultaneous detection of 43 Apoptotic markers in cell and tissue lysates (8 samples). Intended for use with cell and tissue lysates. (catalog #: AAH-APO-G1-8)
References
- Horiuchi T,et al..(2000) Dominant expression of a novel splice variant of caspase-8 in human peripheral blood lymphocytes. Biochem Biophys Res Commun. 272(3):877-81.
- Shivapurkar N, et al..(2002) Differential inactivation of caspase-8 in lung cancers. Cancer Biol Ther. 1(1):65-9.
Expiration:
12 months from the date of shipment when stored properly.