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Anti-Cathepsin D (CTSD) Antibody, Biotinylated
Catalog #: 130-10130B-50
Rabbit anti-Cathepsin D (CTSD) Biotinylated Antibody, 50 ug
Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | AAH16320 |
| Clonality | Polyclonal |
| Immunogen | The immunogen was a recombinant protein. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum followed by Protein A/G affinity chromatography. |
| Isotype | IgG |
| Recommended Applications | ELISA (recommended work dilution= 1:320, 000), Western Blotting (recommended work dilution= 1: 1, 000-5, 000), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, recombinant protein Cathepsin D. The membrane blot was probed with anti- Cathepsin D primary antibody (0.2 µg/ml). Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to IRDye 800CW detection system. Arrows indicate recombinant protein Cathepsin D from E.coli cell (44kDa). |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Cathepsin D (CTSD) a member of aspartyl protease family is a lysosomal endoprotease . This proteinase, which is a member of the peptidase C1 family, has a specificity similar to, but narrower than, that of pepsin A.
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
This antibody binds to its immunogen, and did not show any cross reactivity with unrelated antigens in ELISA. Cross reactivity with mouse and rat Cathepsin D has not been tested yet.
Related Products
Recombinant Human Cathepsin-D (Cat: 228-10292)Mouse Anti-Human Cathepsin D (Cat: DS-MB-00203)Native Human Cathepsin D (Cat: MD-14-0025P)
References
- Sabine Koch, et al. Morphological and Functional Correlates of Synaptic Pathology in the Cathepsin D Knock-Out Mouse Model of Congenital Neuronal Ceroid-Lipofuscinosis. J Neuropathol Exp Neurol. 2011 December; 70(12): 10891096.
- Zinayida Shevtsova, et al. CNS-Expressed Cathepsin D Prevents Lymphopenia in a Murine Model of Congenital Neuronal Ceroid Lipofuscinosis. Am J Pathol. 2010 July; 177(1): 271279.
Expiration:
12 months from the date of shipment when stored properly.