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Anti-Caveolin 2 Antibody
Catalog #: 107-10752
Caveolin 2 antibody, 0.94 mg/ml (100 ul)
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 858 |
| Protein Name / Synonyms | Caveolin 2 |
| Concentration (lot specific) | 0.94 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Synonyms
CAV antibody, MGC12294 antibody, CAV2 antibody, caveolin-2 antibody, caveolin 2 isoform a and b antibody, "caveolae protein, 20-kD antibody", caveolin 2 antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
18 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 1 and 45 of Caveolin 2
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000[suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Caveolin 2 antibody detects Caveolin 2 protein at membrane by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Caveolin 2 protein stained by Caveolin 2 antibody (107-10752) diluted at 1:500.
Blue: Hoechst 33342 staining.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Caveolin 2 protein stained by Caveolin 2 antibody (107-10752) diluted at 1:500.
Blue: Hoechst 33342 staining.
Caveolin 2 antibody immunoprecipitates caveolin 2 protein in IP experiments.
IP samples: A549 whole cell extract
A. 30 µg A549 whole cell extract
B. Control with 4 µg of preimmune Rabbit IgG
C. Immunoprecipitation of caveolin 2 protein by 4 µg Caveolin 2 antibody (107-10752)
15 % SDS-PAGE
The immunoprecipitated caveolin 2 protein was detected by Caveolin 2 antibody (107-10752) diluted at 1:500.
[EasyBlot anti-rabbit IgG was used as a secondary reagent]
IP samples: A549 whole cell extract
A. 30 µg A549 whole cell extract
B. Control with 4 µg of preimmune Rabbit IgG
C. Immunoprecipitation of caveolin 2 protein by 4 µg Caveolin 2 antibody (107-10752)
15 % SDS-PAGE
The immunoprecipitated caveolin 2 protein was detected by Caveolin 2 antibody (107-10752) diluted at 1:500.
[EasyBlot anti-rabbit IgG was used as a secondary reagent]
Immunohistochemical analysis of paraffin-embedded FaDu xenograft, using Caveolin 2(107-10752) antibody at 1:500 dilution.
Sample (30 ug of whole cell lysate)
A: A549
12% SDS PAGE
107-10752 diluted at 1:1000
A: A549
12% SDS PAGE
107-10752 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.