Anti-CBFA2T2 (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	O43439
Gene Id	9139
Gene Symbols	CBFA2T2
Protein Name / Synonyms	CBFA2T2 (N-term)
Clonality	Polyclonal
Preparation	This CBFA2T2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-29 amino acids from the N-terminal region of human CBFA2T2.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	IF, Western Blotting
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

In acute myeloid leukemia, especially in the M2 subtype, the t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 (AML1) gene fused to the 3'-region of the CBFA2T1 (MTG8) gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. The protein encoded by this gene binds to the AML1-MTG8 complex and may be important in promoting leukemogenesis. Several transcript variants are thought to exist for this gene, but the full-length natures of only three have been described.

Specificity

Other Names

CBFA2T2; EHT; MTGR1; Protein CBFA2T2; ETO homologous on chromosome 20; MTG8-like protein; MTG8-related protein 1; Myeloid translocation-related protein 1; p85

NCBI Accession #

NP_001028171.1;NP_001034798.1;NP_005084.1

Other Accession #

NP_001028171.1; NP_005084.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

67133

Recommended Dilutions

IF: 1:10-50; WB: 1:1000

Fluorescent image of Hela cell stained with CBFA2T2 Antibody (N-term)(Cat# 102-12910).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CBFA2T2 primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°).CBFA2T2 immunoreactivity is localized to Nucleus significantly.

CBFA2T2 Antibody (N-term) (Cat# 102-12910) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the CBFA2T2 antibody detected the CBFA2T2 protein (arrow).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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