CBS antibody, 1 mg/ml (100 ul)
Loading...
Loading...
Loading...
Loading...
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 875 |
| Protein Name / Synonyms | CBS |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
HIP4 antibody, CBS antibody, serine sulfhydrase antibody, beta-thionase antibody, cystathionine beta-synthase antibody, methylcysteine synthase antibody, cystathionine-beta-synthase antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
1XPBS, 1%BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
61 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 39 and 251 of CBS (Uniprot ID# P35520)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CBS(107-11060) antibody at 1:200 dilution.
CBS antibody immunoprecipitates CBS protein in IP experiments.
IP samples: HepG2 whole cell extract
A. 50 ?g HepG2 whole cell extract
B. Control with 4 ?g of preimmune Rabbit IgG
C. Immunoprecipitation of CBS protein by 4 ?g CBS antibody (107-11060)
7.5 % SDS-PAGE
The immunoprecipitated CBS protein was detected by CBS antibody (107-11060) diluted at 1:500.
[EasyBlot anti-rabbit IgG was used as a secondary reagent]
IP samples: HepG2 whole cell extract
A. 50 ?g HepG2 whole cell extract
B. Control with 4 ?g of preimmune Rabbit IgG
C. Immunoprecipitation of CBS protein by 4 ?g CBS antibody (107-11060)
7.5 % SDS-PAGE
The immunoprecipitated CBS protein was detected by CBS antibody (107-11060) diluted at 1:500.
[EasyBlot anti-rabbit IgG was used as a secondary reagent]
CBS antibody detects CBS protein by Western blot analysis.
A. 30 µg 293T whole cell lysate/extract
7.5 % SDS-PAGE
CBS antibody (107-11060) dilution: 1:1000
A. 30 µg 293T whole cell lysate/extract
7.5 % SDS-PAGE
CBS antibody (107-11060) dilution: 1:1000
Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft , using CBS(107-11060) antibody at 1:500 dilution.
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.