Anti-CBS (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P35520
Gene Id	875
Gene Symbols	CBS
Protein Name / Synonyms	CBS (N-term)
Clonality	Polyclonal
Preparation	This CBS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104-133 amino acids from the N-terminal region of human CBS.
Concentration (lot specific)	0.290 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF, FC
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

CBS acts as a homotetramer to catalyze the conversion of homocysteine to cystathionine, the first step in the transsulfuration pathway. This protein is allosterically activated by adenosyl-methionine and uses pyridoxal phosphate as a cofactor. Defects in this gene can cause cystathionine beta-synthase deficiency (CBSD), which can lead to homocystinuria.

Specificity

Other Names

CBS; Cystathionine beta-synthase; Beta-thionase; Serine sulfhydrase

NCBI Accession #

NP_000062.1;NP_001171479.1;NP_001171480.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

60587

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; FC: 1:10-50; IF: 1:10-50

	Western blot analysis of CBS Antibody (N-term) (Cat# 102-17038) in mouse kidney tissue and Ramos cell line lysates (35ug/lane). CBS (arrow) was detected using the purified Pab.
	CBS Antibody (N-term) (Cat# 102-17038) IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CBS Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
	CBS Antibody (N-term) (Cat# 102-17038) flow cytometric analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	Western blot analysis of CBS (arrow) using rabbit polyclonal CBS Antibody (N-term) (Cat# 102-17038). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CBS gene.
	Confocal immunofluorescent analysis of CBS Antibody (N-term)(Cat# 102-17038) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).