Rabbit Anti-Human CCK4 (PTK7) (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q13308 |
| Gene Id | 5754 |
| Gene Symbols | PTK7 |
| Protein Name / Synonyms | CCK4 (PTK7) (N-term) |
| Clonality | Polyclonal |
| Preparation | This CCK4 (PTK7) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 21-52 amino acids from the N-terminal region of human CCK4 (PTK7). |
| Concentration (lot specific) | 1.860 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, FC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
CCK4 may function as a cell adhesion molecule. Although it belongs to the insuline receptor subfamily of the Tyr protein kinases, it likely lacks the catalytic activity of a tyrosine kinase. It may be connected to the pathophysiology of colon carcinomas and/or may represent a tumor progression marker. This Type I membrane protein is highly expressed in lung, liver, pancreas, kidney, placenta and melanocytes, but weakly expressed in thyroid gland, ovary, brain, heart and skeletal muscle, and not in colon. It is also expressed in erythroleukemia cells.
Specificity
Other Names
PTK7; CCK4; Inactive tyrosine-protein kinase 7; Colon carcinoma kinase 4; Protein-tyrosine kinase 7; Pseudo tyrosine kinase receptor 7; Tyrosine-protein kinase-like 7
NCBI Accession #
NP_001257327.1;NP_002812.2;NP_690619.1;NP_690620.1;NP_690621.1
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
118392
Recommended Dilutions
WB: 1:1000; FC: 1:10-50; IHC: 1:50-100
 | Western blot analysis of CCK4 (arrow) using rabbit polyclonal CCK4 Antibody (N-term) (Cat# 102-17629). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PTK7 gene (Lane 2) (Origene Technologies). |
 | Flow cytometric analysis of NCI-H460 cells using CCK4 (PTK7) Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
 | Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.