Anti-CCL2 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P13500
Gene Id	6347
Gene Symbols	CCL2
Protein Name / Synonyms	CCL2 (C-term)
Clonality	Polyclonal
Preparation	This CCL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-89 amino acids from the C-terminal region of human CCL2.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF, FC
Research Area	Angiogenesis, Cancer, Inflammation, Chemokine Biology
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. CCL2 is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.

Specificity

Predicted Reactivity

Monkey

Other Names

CCL2; MCP1; SCYA2; C-C motif chemokine 2; HC11; Monocyte chemoattractant protein 1; Monocyte chemotactic and activating factor; Monocyte chemotactic protein 1; Monocyte secretory protein JE; Small-inducible cytokine A2

NCBI Accession #

NP_002973.1

Other Accession #

P61274

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

11025

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50; IF: 1:10-50; FC: 1:10-50

	Western blot analysis of CCL2 Antibody (C-term) (Cat# 102-16896) in Hela cell line lysates (35ug/lane). CCL2 (arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human brain tissue with CCL2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Immunofluorescence analysis of CCL2 Antibody (C-term) with hela cells . 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.
	Confocal immunofluorescent analysis of CCL2 Antibody (C-term) (Cat# 102-16896) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
	Flow cytometric analysis of hela cells using CCL2 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.