Anti-CCNB1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P14635
Gene Id	891
Gene Symbols	CCNB1
Protein Name / Synonyms	CCNB1, CCNB, cyclin B1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

44kDa/48kDa

Observed MW

55kDa

Immunogen

A synthetic peptide of human CCNB1

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using CCNB1 Antibody (144-02056) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Western blot - CCNB1 Polyclonal Antibody (144-02056)

Immunofluorescence analysis of A549 cells using CCNB1 antibody (144-02056). Blue: DAPI for nuclear staining.
Immunofluorescence - CCNB1 Polyclonal Antibody (144-02056)

Expiration:

12 months from the date of shipment when stored properly.

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