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Anti-CD11b (N-term) Antibody
Catalog #: 102-10744
Rabbit Anti-Human CD11b (N-term) Antibody, 400 ul
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P11215 |
| Gene Id | 3684 |
| Gene Symbols | ITGAM |
| Protein Name / Synonyms | CD11b (N-term) |
| Clonality | Polyclonal |
| Preparation | This CD11b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 253-282 amino acids from the N-terminal region of human CD11b. |
| Concentration (lot specific) | 0.270 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, FC |
| Research Area | Inflammation, Autoimmune Disease, Adaptive Immunity |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
CD11b is the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles.
Specificity
Other Names
ITGAM; CD11B; CR3A; Integrin alpha-M; CD11 antigen-like family member B; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; Leukocyte adhesion receptor MO1; Neutrophil adherence receptor; CD_antigen=CD11b; Flags: Precursor
NCBI Accession #
NP_000623.2;NP_001139280.1
Other Accession #
NP_000623
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
127179
Recommended Dilutions
IHC: 1:50-100; FC: 1:10-50; WB: 1:1000
 | CD11b antibody (N-term) (Cat# 102-10744) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CD11b antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated. |
 | CD11b Antibody (N-term) (Cat# 102-10744) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
 | CD11b Antibody (N-term) (Cat# 102-10744) western blot analysis in A549,K562 cell line lysates (35ug/lane).This demonstrates the CD11b antibody detected the CD11b protein (arrow). |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.