Anti-CD138 (C-term) (Ascites) Antibody

Product Description

Specifications

Size	0.1 mL
Estimated Lead Time	1-2 weeks
Species	Human
Accession Number	P18827
Gene Id	6382
Gene Symbols	SDC1
Protein Name / Synonyms	CD138 (C-term) (Ascites)
Clonality	Monoclonal
Preparation	This CD138 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 210-238 amino acids from the C-terminal region of human CD138.
Recommended Applications	Western Blotting, FC, IF
Research Area	Adaptive Immunity
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Altered syndecan-1 expression has been detected in several different tumor types. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same protein.

Specificity

Other Names

SDC1; SDC; Syndecan-1; CD_antigen=CD138; Flags: Precursor

NCBI Accession #

NP_001006947.1;NP_002988.3

Other Accession #

NP_001006947.1

Format

Type

Mouse Monoclonal Antibody (Mab)

Calculated Molecular Weight (Da)

32462

Recommended Dilutions

WB: 1:500-8000; FC: 1:10-50; IF: 1:10-50

	CD138 Antibody (C-term)(Ascites)(Cat# 102-10366) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the CD138 antibody detected the CD138 protein (arrow).
	CD138 Antibody (C-term) (Ascites) (Cat# 102-10366) flow cytometric analysis of U266 cells (right histogram) compared to a negative control cell (left histogram).Alexa Fluor 488-conjugated donkey anti-mouse lgG secondary antibodies were used for the analysis
	CD138 Antibody (C-term) (Ascites) (Cat# 102-10366) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).Alexa Fluor 488-conjugated donkey anti-mouse lgG secondary antibodies were used for the analysis
	CD138 Antibody (C-term) (Ascites) (Cat# 102-10366) flow cytometric analysis of T47D cells (right histogram) compared to a negative control cell (left histogram).Alexa Fluor 488-conjugated donkey anti-mouse lgG secondary antibodies were used for the analysis
	Confocal immunofluorescent analysis of CD138 Antibody (C-term) (Ascites)(Cat# 102-10366) with U266 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
	Confocal immunofluorescent analysis of CD138 Antibody (C-term) (Ascites)(Cat# 102-10366) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
	Confocal immunofluorescent analysis of CD138 Antibody (C-term) (Ascites)(Cat# 102-10366) with T47D cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).