Anti-CD14 (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P08571
Gene Id	929
Gene Symbols	CD14
Protein Name / Synonyms	CD14 (N-term)
Clonality	Polyclonal
Preparation	This CD14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 54-83 amino acids from the N-terminal region of human CD14.
Concentration (lot specific)	2.000 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF, FC
Research Area	Cancer, Inflammation, Adaptive Immunity
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

CD14 is a surface protein preferentially expressed on monocytes/macrophages. It binds lipopolysaccharide binding protein and recently has been shown to bind apoptotic cells.

Specificity

Other Names

CD14; Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein; CD_antigen=CD14; Monocyte differentiation antigen CD14, urinary form; Monocyte differentiation antigen CD14, membrane-bound form

NCBI Accession #

NP_000582.1;NP_001035110.1;NP_001167575.1;NP_001167576.1

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

40076

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50; IF: 1:10-50; FC: 1:10-50

	Western blot analysis of CD14(arrow) using rabbit polyclonal CD14 Antibody (N-term) (Cat# 102-16732). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD14 gene (Lane 2) (Origene Technologies).
	CD14 Antibody (N-term) (Cat# 102-16732) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the CD14 antibody detected the CD14 protein (arrow).
	Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CD14 antibody (N-term) (Cat# 102-16732), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Confocal immunofluorescent analysis of CD14 Antibody (N-term)(Cat# 102-16732) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
	CD14 Antibody (N-term) (Cat# 102-16732) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.