Anti-CD19 (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P15391
Gene Id	930
Gene Symbols	CD19
Protein Name / Synonyms	CD19 (N-term)
Clonality	Polyclonal
Preparation	This CD19 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 143-172 amino acids from the N-terminal region of human CD19.
Concentration (lot specific)	0.500 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF, FC
Research Area	Adaptive Immunity
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. CD19 is a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.

Specificity

Other Names

CD19; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; Differentiation antigen CD19; T-cell surface antigen Leu-12; CD_antigen=CD19; Flags: Precursor

NCBI Accession #

NP_001171569.1;NP_001761.3

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

61128

Recommended Dilutions

WB: 1:1000; IF: 1:10-50; FC: 1:10-50; IHC: 1:10-50

	CD19 Antibody (N-term) (Cat# 102-12737) western blot analysis in Raji cell line lysates (35ug/lane).This demonstrates the CD19 antibody detected the CD19 protein (arrow).
	Western blot analysis of CD19 (arrow) using rabbit polyclonal CD19 Antibody (N-term) (Cat# 102-12737). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD19 gene (Lane 2) (Origene Technologies).
	Immunofluorescence analysis of CD19 Antibody (N-term) with paraffin-embedded human lymph tissue . 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.
	Flow cytometric analysis of CEM cells using CD19 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	Formalin-fixed and paraffin-embedded human lymph reacted with CD19 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.