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Anti-CD20 Antibody, PE
Catalog #: 188-10032-PE
Mouse anti-Human CD20 Antibody [PE Conjugated]
Product Description
Specifications
| Size | 100 tests |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Specificity | Other species not known |
| Host Species | Mouse |
| Accession Number | P11836 |
| Gene Id | 931 |
| Gene Symbols | MS4A1,CD20 |
| Protein Name / Synonyms | B-lymphocyte antigen CD20 (B-lymphocyte surface antigen B1) (Bp35) (Leukocyte surface antigen Leu-16) (Membrane-spanning 4-domains subfamily A member 1) (CD antigen CD20) |
| Immunogen | The lymphoblastoid cell line Daudi was used as the immunogen for this CD20 antibody. |
| Isotype | Kappa, IgG2a |
| Purity | Protein G affinity chromatography |
| Concentration (lot specific) | 500 ul at 0.1 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
| Recommended Applications | Flow Cytometry: 5ul per test per one million cells in 0.1ml (or 5ul per 100ul of whole blood) Immunofluorescence: 1:50-1:100 |
| Domain / Region / Terminus | Predominantly cell surface with some cytoplasmic |
| Research Area | Adaptive Immunity |
| Shipping Type | Blue ice |
| Storage | 2-8°C |
Description
Description
This antibody recognizes a protein of 33-37kDa, identified as CD20 (Workshop V; Code CD20.12). The antibody recognizes the extracellular domain of CD20. The epitope is similar to or identical to that recognized by other CD20 antibodies including Leu-16 and B1. This antibody can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood, B cell localization in tissues and B lymphocyte purification by immunosorbent methods. CD20 is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. Protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. CD20 is not glycosylated in resting B cells and its cytoplasmic domains are differentially phosphorylated upon activation. It acts as a calcium channel involved in B-cell activation and cell cycle progression.
Storage
Store the CD20 antibody at 2-8°C. The conjugate is light-sensitive, protect from light.
Expiration:
12 months from the date of shipment when stored properly.