Anti-CD4 (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P01730
Gene Id	920
Gene Symbols	CD4
Protein Name / Synonyms	CD4 (N-term)
Clonality	Polyclonal
Preparation	This CD4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 58-86 amino acids from the N-terminal region of human CD4.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, FC
Research Area	Adaptive Immunity
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes (helper/inducer T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.

Specificity

Other Names

CD4; T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3; CD_antigen=CD4; Flags: Precursor

NCBI Accession #

NP_000607.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

51111

Recommended Dilutions

WB: 1:1000; FC: 1:10-50

	Western blot analysis of CD4 Antibody (N-term) in CEM cell line lysates (35ug/lane). CD4(arrow) was detected using the purified Pab.
	Western blot analysis of CD4 (arrow) using rabbit polyclonal CD4 Antibody (N-term) (RB12862). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD4 gene (Lane 2) (Origene Technologies).
	CD4 Antibody (N-term) (Cat# 102-12749) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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