Anti-CD45 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P08575
Gene Id	5788
Gene Symbols	PTPRC
Protein Name / Synonyms	CD45 (C-term)
Clonality	Polyclonal
Preparation	This CD45 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1245-1275 amino acids from the C-terminal region of human CD45.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC
Research Area	Cancer, Inflammation, Autoimmune Disease, Adaptive Immunity
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

CD45 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus belongs to receptor type PTP. The CD45 gene is specifically expressed in hematopoietic cells. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling.

Specificity

Other Names

PTPRC; CD45; Receptor-type tyrosine-protein phosphatase C; Leukocyte common antigen; T200; CD_antigen=CD45; Flags: Precursor

NCBI Accession #

NP_002829.3;NP_563578.2

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

147254

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50; FC: 1:10-50

	The anti-CD45 (C-term) Pab (Cat# 102-12895) is used in Western blot to detect CD45 in jurkat cell lysate.
	Formalin-fixed and paraffin-embedded human tonsil reacted with CD45 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Flow cytometric analysis of jurkat cells using CD45 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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