Anti-CD46 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P15529
Gene Id	4179
Gene Symbols	CD46
Protein Name / Synonyms	CD46 (C-term)
Clonality	Polyclonal
Preparation	This CD46 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 317-343 amino acids from the C-terminal region of human CD46.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC, IF
Research Area	Adaptive Immunity
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

CD46 is a type I membrane protein and is a regulatory part of the complement system. The encoded protein has cofactor activity for inactivation of complement components C3b and C4b by serum factor I, which protects the host cell from damage by complement. In addition, the encoded protein can act as a receptor for the Edmonston strain of measles virus, human herpesvirus-6, and type IV pili of pathogenic Neisseria. Finally, the protein encoded by this gene may be involved in the fusion of the spermatozoa with the oocyte during fertilization. This gene is found in a cluster on chromosome 1q32 with other genes encoding structural components of the complement system.

Specificity

Other Names

CD46; MCP; MIC10; Membrane cofactor protein; TLX; Trophoblast leukocyte common antigen; CD_antigen=CD46; Flags: Precursor

NCBI Accession #

NP_002380.3;NP_722548.1;NP_758860.1;NP_758861.1;NP_758862.1;NP_758863.1;NP_758869.1;NP_758871.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

43747

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; FC: 1:10-50; IF: 1:10-50

	Western blot analysis of lysates from Jurkat,SK-BR-3 cell line (from left to right),using CD46 Antibody (C-term)(Cat# 102-15761).102-15761 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.
	CD46 Antibody (C-term) (Cat# 102-15761) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CD46 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
	CD46 Antibody (C-term) (Cat# 102-15761) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	Confocal immunofluorescent analysis of CD46 Antibody (C-term) (Cat# 102-15761) with Hela cell followed by Alexa Fluor^® 489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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