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Anti-CD5 Antibody
Catalog #: 130-10152
Rabbit Anti- Cluster of differentiation 5 (CD5) Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | AAH27901 |
| Clonality | Polyclonal |
| Immunogen | The immunogen was a recombinant protein. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum followed by Protein A/G affinity chromatography. |
| Recommended Applications | ELISA (recommended work dilution= 1: 16, 000), Western Blotting (recommended work dilution= 1: 1, 000-5, 000), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, recombinant protein CD5. The membrane blot was probed with anti-CD5primary antibody (0.2 µg/ml). Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to IRDye 800CW detection system. Arrows indicate recombinant protein CD5from E.coli cell (38kDa). |
| Antigen Catalog Number | 230-00244 |
| Reconstitution | Product is supplied as a powder obtained from lyophilization of purified antibody in PBS without preservatives. Reconstitute the antibody with sterile water to a final concentration of 1 mg/ml. |
| Research Area | Adaptive Immunity |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
CD5 is a cluster of differentiation found on a subset of IgM-secreting B cells called B-1 cells, and also on T cells, which can play a role as a regulator of cell death and as a receptor for pathogen associated molecular patterns.
Specificity
This antibody binds to its immunogen, and did not show any cross reactivity with unrelated antigens in ELISA. Cross reactivity with mouse and rat CD5 has not been tested yet.
Storage
Store at 4°C if intended for use within one month, otherwise, store at -20°C to -80°C. The lyophilized antibody is stable for at least 18 months after the date of receipt when stored at -20°C to -80°C. After reconstitution, it can be aliquoted and stored frozen at -20°C to -80°C in a manual defrost freezer for at least 6 months without detectable loss of activity. Upon reconstitution, the antibody can also be stored for 1 month at 4°C. Please avoid freeze-thaw cycles, as this will lower the activity of the antibody.
Related Products
Mouse Anti-Bovine CD5 (Cat: DS-MB-02634) Mouse Anti-Chicken CD5 (Cat: DS-MB-02693) Mouse Anti-Human CD5 [+RPE-Cy5] (Cat: DS -MB-03125)
References
- Gloria Soldevila, et al. The immunomodulatory properties of the CD5 lymphocyte receptor in health and disease. Curr Opin Immunol. 2011 June; 23(3): 310318.
- Mohammed A. Sarhan,et al. Hepatitis C Virus Infection of Human T Lymphocytes Is Mediated by CD5. J Virol. 2012 April; 86(7): 37233735.
Expiration:
12 months from the date of shipment when stored properly.