Anti-CD73 (NT5E) (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P21589
Gene Id	4907
Gene Symbols	NT5E
Protein Name / Synonyms	CD73 (NT5E) (C-term)
Clonality	Polyclonal
Preparation	This CD73 (NT5E) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 520-550 amino acids from the C-terminal region of human CD73 (NT5E).
Concentration (lot specific)	2.000 mg/ml
Recommended Applications	Western Blotting, IHC-P
Research Area	Inflammation, Adaptive Immunity
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70 kD subunits bound externally to the plasma membrane by a glycosyl phosphatidyl inositol linkage. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5E occurs in a variety of immunodeficiency diseases. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. It is not known whether the different enzymes are coded by different genes or result from different posttranslational modifications of a single coding sequence.

Specificity

Other Names

NT5E; NT5; NTE; 5'-nucleotidase; Ecto-5'-nucleotidase; CD_antigen=CD73; Flags: Precursor

NCBI Accession #

NP_001191742.1;NP_002517.1

Other Accession #

NP_002517

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

63368

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50

	Western blot analysis of CD73 Pab (Cat# 102-14569) in mouse brain tissue lysate. CD73 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
	Western blot analysis of NT5E (arrow) using rabbit polyclonal NT5E Antibody (M535) (Cat# 102-14569). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NT5E gene.
	Formalin-fixed and paraffin-embedded human Placenta tissue reacted with CD73 (NT5E) Antibody (C-term)(Cat# 102-14569), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products