Anti-CD74 (N-term) Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Gene Id	972
Protein Name / Synonyms	CD74, N-term
Concentration (lot specific)	0.88 mg/ml
Recommended Applications	IHC (Formalin-fixed paraffin-embedded sections), Western blot
Research Area	Adaptive Immunity
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

DHLAG antibody, HLADG antibody, II antibody, Ia-GAMMA antibody, CD74 antibody, MHC HLA-DR gamma chain antibody, HLA-DR antigens-associated invariant chain antibody, HLA-DR-gamma antibody, p33 antibody, HLA class II histocompatibility antigen gamma chain antibody, CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) antibody, gamma chain of class II antigens antibody, Ia-associated invariant chain antibody, CD74 molecule, major histocompatibility complex, class II invariant chain antibody

Specificity

Species Reactivity

Human, Rat, Mouse

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

34 kDa

Immunogen

Synthetic peptide corresponding to a region within amino acids 1 and 58 of CD74 (Uniprot ID# P04233)

Applications

Applications Tested

IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

CD74 antibody [N1N2], N-term detects CD74 protein by Western blot analysis.
A. 30 µg GL261 whole cell lysate/extract
12 % SDS-PAGE
CD74 antibody [N1N2], N-term (107-10917) dilution: 1:1000

CD74 antibody [N1N2], N-term detects CD74 protein by Western blot analysis.
A. 30 µg Rat2 whole cell lysate/extract
12 % SDS-PAGE
CD74 antibody [N1N2], N-term (107-10917) dilution: 1:1000

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using CD74(107-10917) antibody at 1:500 dilution.

Sample (30 ug of whole cell lysate)
A: Raji
12% SDS PAGE
107-10917 diluted at 1:5000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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