RayBiotech

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ISO 13485:2016 cGMP

Anti-CD99 Antibody

Catalog #: 144-02028

CD99 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman
Host SpeciesRabbit
Accession Number
P14209
Gene Id
4267
Gene Symbols
CD99
Protein Name / Synonyms
CD99, HBA71, MIC2, MIC2X, MIC2Y, MSK5X, CD99 antigen
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.09% Sodium azide, 50% glycerol, pH 7.3
Recommended ApplicationsWestern Blotting
Research AreaAdaptive Immunity
Shipping TypeBlue ice
Storage-20°C

Description

Background

The protein encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. There is a related pseudogene located immediately adjacent to this locus.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW
16kDa/17kDa/18kDa
Observed MW
22kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 23-185 of human CD99 (NP_002405.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using CD99 antibody (144-02028) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - CD99 antibody (144-02028)

Expiration:
12 months from the date of shipment when stored properly.