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Anti-CDC2 (N-term) Antibody
Catalog #: 102-12755
Rabbit Anti-Human CDC2 (N-term) Antibody, 400 ul
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P06493 |
| Gene Id | 983 |
| Gene Symbols | CDK1 |
| Protein Name / Synonyms | CDC2 (N-term) |
| Clonality | Polyclonal |
| Preparation | This CDC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-29 amino acids from the N-terminal region of human CDC2. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting, FC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
CDC2 is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control.
Specificity
Predicted Reactivity
Bovine, Chicken
Other Names
CDK1; CDC2; CDC28A; CDKN1; P34CDC2; Cyclin-dependent kinase 1; Cell division control protein 2 homolog; Cell division protein kinase 1; p34 protein kinase
NCBI Accession #
NP_001777.1;NP_203698.1
Other Accession #
P13863; P48734
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
34095
Recommended Dilutions
WB: 1:1000; FC: 1:10-50
 | Western blot analysis of CDC2 Antibody (N-term) in 293 cell line lysates (35ug/lane). CDC2 (arrow) was detected using the purified Pab. |
 | Western blot analysis of CDC2 (arrow) using rabbit polyclonal CDC2 Antibody (N-term) (Cat# 102-12755).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDC2 gene (Lane 2) (Origene Technologies). |
 | Flow cytometric analysis of 293 cells using CDC2 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.