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Anti-CDC25B (Center) Antibody
Catalog #: 102-17216
Rabbit Anti-Human CDC25B (Center) Antibody, 400 ul
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P30305 |
| Gene Id | 994 |
| Gene Symbols | CDC25B |
| Protein Name / Synonyms | CDC25B (Center) |
| Clonality | Polyclonal |
| Preparation | This CDC25B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 331-360 amino acids from the Central region of human CDC25B. |
| Concentration (lot specific) | 0.396 mg/ml |
| Recommended Applications | Western Blotting, IHC-P |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined.
Specificity
Other Names
CDC25B; CDC25HU2; M-phase inducer phosphatase 2; Dual specificity phosphatase Cdc25B
NCBI Accession #
NP_004349.1;NP_068658.1;NP_068659.1
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
64987
Recommended Dilutions
WB: 1:1000; IHC: 1:10-50
 | Western blot analysis of anti-CDC25B Antibody (Center)(Cat# 7256c) in Ramos cell line lysates (35ug/lane). CDC25B(arrow) was detected using the purified Pab. |
 | Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with CDC25B antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.