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Anti-CDCA8 (Center) Antibody
Catalog #: 102-12501
Rabbit Anti-Human CDCA8 (Center) Antibody, 400 ul
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q53HL2 |
| Gene Id | 55143 |
| Gene Symbols | CDCA8 |
| Protein Name / Synonyms | CDCA8 (Center) |
| Clonality | Polyclonal |
| Preparation | This CDCA8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 115-144 amino acids from the Central region of human CDCA8. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, FC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
CDCA8 is a component of the chromosomal passenger complex (CPC) required for stability of the bipolar mitotic spindle. The CPC complex, a key regulator of mitosis, has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. In the complex, CDCA8 may be required to direct the CPC to centromeric DNA.
Specificity
Other Names
CDCA8; PESCRG3; Borealin; Dasra-B; Cell division cycle-associated protein 8; Pluripotent embryonic stem cell-related gene 3 protein
NCBI Accession #
NP_001243804.1;NP_060571.1
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
31323
Recommended Dilutions
WB: 1:1000; IHC: 1:10-50; FC: 1:10-50
 | Western blot analysis of CDCA8 Antibody (Center) (Cat# 102-12501) in HL60 cell line lysates (35ug/lane). CDCA8(arrow) was detected using the purified polyclonal antibody. |
 | Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CDCA8 antibody (Center) (Cat# 102-12501), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | Flow cytometric analysis of HL-60 cells using CDCA8 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.