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Anti-CDK4 Antibody
Catalog #: 102-20154
Mouse Anti-CDK4 Antibody, 200 µl
Product Description
Specifications
| Size | 200 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Mouse |
| Accession Number | P11802 |
| Gene Id | 1019 |
| Gene Symbols | CDK4 |
| Protein Name / Synonyms | Cyclin-dependent kinase 4, Cell division protein kinase 4, PSK-J3, CDK4 |
| Clonality | Monoclonal |
| Clone | 1529CT850.162.73 |
| Isotype | Kappa, IgG2a |
| Recommended Applications | IHC-P, Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
Other Information
Format
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Calculated Molecular Weight (Da)
33730
Antigen Source
Human
Images
IHC-P (1:25)
102-20154 staining CDK4 in human breast carcinoma sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.