Anti-CDK5R1 (p35) (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q15078
Gene Id	8851
Gene Symbols	CDK5R1
Protein Name / Synonyms	CDK5R1(p35) (C-term)
Clonality	Polyclonal
Preparation	This CDK5R1(p35) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 272-301 amino acids from the C-terminal region of human CDK5R1(p35).
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	IHC-P, Western Blotting
Research Area	Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

p35 is a neuron-specific activator of cyclin-dependent kinase 5 (CDK5); the activation of CDK5 is required for proper development of the central nervous system. The p35 form of this protein is proteolytically cleaved by calpain, generating a p25 form. The cleavage of p35 into p25 results in relocalization of the protein from the cell periphery to nuclear and perinuclear regions. P25 deregulates CDK5 activity by prolonging its activation and changing its cellular location. The p25 form accumulates in the brain neurons of patients with Alzheimer's disease. This accumulation correlates with an increase in CDK5 kinase activity, and may lead to aberrantly phosphorylated forms of the microtubule-associated protein tau, which contributes to Alzheimer's disease.

Specificity

Predicted Reactivity

Mouse, Rat, Bovine

Other Names

CDK5R1; CDK5R; NCK5A; Cyclin-dependent kinase 5 activator 1; Cyclin-dependent kinase 5 regulatory subunit 1; TPKII regulatory subunit; Cyclin-dependent kinase 5 activator 1, p35; Cyclin-dependent kinase 5 activator 1, p25; Tau protein kinase II 23 kDa subunit

NCBI Accession #

NP_003876.1

Other Accession #

P61810; P61809; Q28199

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

34060

Recommended Dilutions

IHC: 1:10-50; WB: 1:1000

	Formalin-fixed and paraffin-embedded human brain tissue reacted with CDK5R1(p35) Antibody (C-term) (Cat# 102-17596), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	CDK5R1 Antibody (C-term) (Cat# 102-17596) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the CDK5R1 antibody detected the CDK5R1 protein (arrow).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products