Anti-CDX2 (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q99626
Gene Id	1045
Gene Symbols	CDX2
Protein Name / Synonyms	CDX2 (N-term)
Clonality	Polyclonal
Preparation	This CDX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human CDX2.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

The caudal type homeo box transcription factors 1 (CDX1) and 2 (CDX2) are candidates for directing intestinal development, differentiation, and maintenance of the intestinal phenotype. CDX1 and CDX2 expression is widely present in the human intestinal and colonic mucosae, but not in the gastric mucosa, suggesting a possible role in the terminal differentiation of the intestine. Increased CDX2 expression is associated with chronic atrophic gastritis. Detectable expression of CDX2 precedes expression of CDX1 during the progression of intestinal metaplasia, thus expression of CDX2 may trigger the initiation and development of intestinal metaplasia. Markedly reduced or absent CDX2 expression was noted by immunohistochemistry in 13 of 15 (87%) large cell minimally differentiated carcinomas (LCMDCs), whereas only 1 of the 25 (4%) differentiated adenocarcinomas (DACs) showed reduced CDX2 expression. Thus, a significant decrease in human CDX1 and/or CDX2 expression may be associated with colorectal tumorigenesis.

Specificity

Predicted Reactivity

Mouse

Other Names

CDX2; CDX3; Homeobox protein CDX-2; CDX-3; Caudal-type homeobox protein 2

NCBI Accession #

NP_001256.3

Other Accession #

P43241

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

33520

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100

	Western blot analysis of CDX2(arrow) using rabbit polyclonal CDX2 Antibody (N-term)(Cat# 102-16651). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDX2 gene (Lane 2) (Origene Technologies).
	The anti-CDX2 N-term Pab (Cat# 102-16651) is used in Western blot to detect CDX2 in placenta tissue lysate.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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