Synonyms
CENTA1 antibody, GCS1L antibody, p42IP4 antibody, ADAP1 antibody, cnt-a1 antibody, putative MAPK-activating protein PM25 antibody, arf-GAP with dual PH domain-containing protein 1 antibody, centaurin-alpha-1 antibody, "centaurin, alpha 1 antibody", centaurin-alpha antibody, ArfGAP with dual PH domains 1 antibody
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
43 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 312 and 374 of Centaurin alpha 1 (Uniprot ID# O75689)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunohistochemical analysis of paraffin-embedded gastric tissue, using Centaurin alpha 1(107-10621) antibody at 1:100 dilution.

Sample(30 µg of whole cell lysate)
A: A431
B: HeLaS3
7.5% SDS PAGE
107-10621 diluted at 1:500
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.