RayBiotech

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ISO 13485:2016 cGMP

Anti-CHGA Antibody

Catalog #: 144-61314

CHGA Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse
Host SpeciesRabbit
Accession Number
P10645
Gene Id
1113
Gene Symbols
CHGA
Protein Name / Synonyms
CHGA, CGA, chromogranin-A
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IHC
Research AreaCardiovascular Disease
Shipping TypeBlue ice
Storage-20°C

Description

Background

The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active peptides; vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Two other peptides, catestatin and chromofungin, have antimicrobial activity and antifungal activity, respectively. Two transcript variants encoding different isoforms have been found for this gene.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200

Format

Calculated MW
50kDa
Observed MW
50kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 230-380 of human CHGA (NP_001266.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using CHGA antibody (144-61314) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - CHGA antibody (144-61314)

Immunohistochemistry of paraffin-embedded mouse brain using CHGA antibody (144-61314) at dilution of 1:150 (40x lens).
Immunohistochemistry - CHGA antibody (144-61314)

Immunohistochemistry of paraffin-embedded mouse spleen using CHGA antibody (144-61314) at dilution of 1:150 (40x lens).
Immunohistochemistry - CHGA antibody (144-61314)

Expiration:
12 months from the date of shipment when stored properly.