RayBiotech

3607 Parkway Ln Ste 200 Norcross, Georgia 30092

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ISO 13485:2016 cGMP

Anti-CHIA Antibody

Catalog #: 144-61364

CHIA Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
Q9BZP6
Gene Id
27159
Gene Symbols
CHIA
Protein Name / Synonyms
CHIA, AMCASE, CHIT2, TSA1902, chitinase, acidic
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IHC, IF
Research AreaInflammation
Shipping TypeBlue ice
Storage-20°C

Description

Background

The protein encoded by this gene degrades chitin, which is found in the cell wall of most fungi as well as in arthropods and some nematodes. The encoded protein can also stimulate interleukin 13 expression, and variations in this gene can lead to asthma susceptibility. Several transcript variants encoding a few different isoforms have been found for this gene.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW
33kDa/40kDa/52kDa
Observed MW
52kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-368 of human CHIA (NP_001244932.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using CHIA antibody (144-61364) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Western blot - CHIA Polyclonal Antibody (144-61364)

Immunohistochemistry of paraffin-embedded human liver cancer using CHIA antibody (144-61364) at dilution of 1:100 (40x lens).
Immunohistochemistry - CHIA Polyclonal Antibody (144-61364)

Immunohistochemistry of paraffin-embedded human liver injury using CHIA antibody (144-61364) at dilution of 1:100 (40x lens).
Immunohistochemistry - CHIA Polyclonal Antibody (144-61364)

Immunohistochemistry of paraffin-embedded human esophageal cancer using CHIA antibody (144-61364) at dilution of 1:100 (40x lens).
Immunohistochemistry - CHIA Polyclonal Antibody (144-61364)

Immunohistochemistry of paraffin-embedded human gastric cancer using CHIA antibody (144-61364) at dilution of 1:100 (40x lens).
Immunohistochemistry - CHIA Polyclonal Antibody (144-61364)

Expiration:
12 months from the date of shipment when stored properly.