Anti-CHUK Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	O15111
Gene Id	1147
Gene Symbols	CHUK
Protein Name / Synonyms	CHUK, IKBKA, IKK-alpha, IKK1, IKKA, NFKBIKA, TCF16, conserved helix-loop-helix ubiquitous kinase
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF, IP
Research Area	Cancer, Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200

Format

Calculated MW

84kDa

Observed MW

94kDa

Immunogen

A synthetic peptide of human CHUK

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using CHUK antibody (144-02062) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - CHUK Polyclonal Antibody (144-02062)

Immunohistochemistry of paraffin-embedded rat brain using CHUK antibody (144-02062) at dilution of 1:100 (40x lens).
Immunohistochemistry - CHUK Polyclonal Antibody (144-02062)

Immunohistochemistry of paraffin-embedded mouse kidney using CHUK antibody (144-02062) at dilution of 1:100 (40x lens).
Immunohistochemistry - CHUK Polyclonal Antibody (144-02062)

Immunofluorescence analysis of A549 cells using CHUK antibody (144-02062). Blue: DAPI for nuclear staining.
Immunofluorescence - CHUK Polyclonal Antibody (144-02062)

Immunoprecipitation analysis of 200ug extracts of 293T cells using 3ug CHUK antibody (144-02062). Western blot was performed from the immunoprecipitate using CHUK antibody (144-02062) at a dilition of 1:1000.
Immunoprecipitation - CHUK Polyclonal Antibody (144-02062)

Expiration:

12 months from the date of shipment when stored properly.

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