CIP29 antibody [N1C3], 0.33 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 84324 |
| Protein Name / Synonyms | CIP29 |
| Concentration (lot specific) | 0.33 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
CIP29 antibody, HCC1 antibody, HSPC316 antibody, MGC14726 antibody, THO1 antibody, SARNP antibody, nuclear protein Hcc-1 antibody, SAP domain-containing ribonucleoprotein antibody, cytokine-induced protein of 29 kDa antibody, hepatocellular carcinoma 1 antibody, cytokine induced protein 29 kDa antibody, proliferation-associated cytokine-inducible protein CIP29 antibody, proliferation associated cytokine-inducible protein CIP29 antibody, SAP domain containing ribonucleoprotein antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
24 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 4 and 210 of CIP29 (Uniprot ID# P82979)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
CIP29 antibody [N1C3] detects CIP29 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CIP29 protein stained by CIP29 antibody [N1C3] (107-10634) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CIP29 protein stained by CIP29 antibody [N1C3] (107-10634) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Immunohistochemical analysis of paraffin-embedded MDAMB-468 xenograft, using CIP29(107-10634) antibody at 1:100 dilution.
Sample(30 ug whole cell lysate)
A:293T
B:A431
C:H1299
12% SDS PAGE
107-10634 diluted at 1:1000
A:293T
B:A431
C:H1299
12% SDS PAGE
107-10634 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.